Projects

INTERVENTION STRATEGIES TO CONTROL AND PREVENT DISEASE OUTBREAKS CAUSED BY AVIAN INFLUENZA AND OTHER EMERGING POULTRY PATHOGENS Appropriated (D)   Accession Number: 422154

INTERVENTION STRATEGIES TO CONTROL NEWCASTLE DISEASE Appropriated (D)   Accession Number: 422161

DEVELOPMENT OF CANARYPOX BASED VACCINES Nonfunded Cooperative Agreement (N)   Accession Number: 421483

FOLLOW-ON: REDUCING DISEASE IN LIVESTOCK Reimbursable (R)   Accession Number: 421570

PATHOGENESIS OF SELECTED NEWCASTLE DISEASE VIRUS FIELD ISOLATES AND RECOMBINANTS Specific Cooperative Agreement (S)   Accession Number: 421212

PATHOGENIC CHARACTERIZATION NEWCASTLE DISEASE VIRUS FIELD ISOLATES Specific Cooperative Agreement (S)   Accession Number: 423209

Pathogenic Characterization Newcastle Disease Virus Field Isolates Specific Cooperative Agreement (S)   Accession Number: 424300

IMPROVEMENT OF NEWCASTLE DISEASE VIRUS VACCINES FOR AFRICA Trust (T)   Accession Number: 421319

DEVELOPMENT OF CANARYPOX BASED VACCINES AGAINST MEXICAN VIRULENT NEWCASTLE DISEASE AND AVIAN INFLUENZA VIRUSES Trust (T)   Accession Number: 421505

EVALUATION OF NEWCASTLE DISEASE VIRUS VACCINES AGAINST CIRCULATING VIRUSES Trust (T)   Accession Number: 421041

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Experimental risk assessment of recombinant Newcastle disease virus vaccines - (Abstract Only) - (30-Dec-12)

Recombinant Newcastle disease vaccines: risk for reversion to virulence and spread in non-target species - (Abstract Only) - (30-Oct-12)

Genetic diversity of avian paramyxovirus type 1: Proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes - (Peer Reviewed Journal)

Diel, D.G., Da Silva, L.A., Liu, H., Wang, Z., Miller, P.J., Afonso, C.L. 2012. Genetic diversity of avian paramyxovirus type 1: Proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes. Infection, Genetics and Evolution. 12(8):1770-1779.

Experimental co-infection studies with avian influenza viruses and Newcastle Disease viruses in chickens, turkeys and domestic ducks - (Abstract Only)

Pantin Jackwood, M.J., Miller, P.J., Afonso, C.L., Spackman, E., Kapczynski, D.R., Shepherd, E.M., Smith, D.M., Cha, R., Sa E Silva, M., Swayne, D.E. 2012. Experimental co-infection studies with avian influenza viruses and Newcastle Disease viruses in chickens, turkeys and domestic ducks [abstract]. American Association of Veterinary Laboratory Diagnosticians. p.139.

Complete genome sequencing of a novel Newcastle disease virus isolate circulating in chicken layers in the Dominican Republic - (Peer Reviewed Journal)

Courtney, S.C., Gomez, D., Killian, M.L., Pedersen, J.C., Miller, P.J., Afonso, C.L. 2012. Complete genome sequencing of a novel Newcastle disease virus isolate circulating in chicken layers in the Dominican Republic. Journal of Virology. 86(17):9550.

Experimental clinical and pathologic characterization of West African Newcastle disease viruses - (Abstract Only)

Jones, M.E., Cattoli, G., Cardenas-Garcia, S., Miller, P.J., Afonso, C.L., Brown, C.C. 2012. Experimental clinical and pathologic characterization of West African Newcastle disease viruses [abstract]. American Association of Avian Pathologists. p.22.

Characterization of the live LaSota-vaccine strain-induced protection in chickens upon early challenge with a virulent Newcastle disease virus of heterologous genotype - (Peer Reviewed Journal)

Edwards, I.C., Miller, P.J., Afonso, C.L. 2012. Characterization of the live LaSota-vaccine strain-induced protection in chickens upon early challenge with a virulent Newcastle disease virus of heterologous genotype. Avian Diseases. 56:464-470.

Genetic diversity of avian paramyxovirus type 1: defining Newcastle disease virus genotypes - (Abstract Only)

Diel, D.G., Da Silva, L.H., Miller, P.J., Afonso, C.L. 2012. Genetic diversity of avian paramyxovirus type 1: defining Newcastle disease virus genotypes [abstract]. American Association of Avian Pathologists. p.22.

Biological and phylogenetic characterization of a genotype VII Newcastle disease virus from Venezuela: Efficacy of vaccination - (Peer Reviewed Journal)

Perozo, F., Marcano, R., Afonso, C.L. 2012. Biological and phylogenetic characterization of a genotype VII Newcastle disease virus from Venezuela: Efficacy of vaccination. Journal of Clinical Microbiology. 50:1204-1208.

Analysis of the genome of the sexually transmitted insect virus Hz-2V - (Peer Reviewed Journal)

Burand, J.P., Kim, W., Afonso, C.L., Tulman, E., Kutish, G.F., Rock, D.L. 2012. Analysis of the genome of the sexually transmitted insect virus Hz-2V. Viruses. 4(1):28-61.

Experimental characterization of West African Newcastle disease virus - (Abstract Only)

Brown, C.C., Jones, M.E., Cattoli, G., Cardenas-Garcia, S., Miller, P.J., Afonso, C.L. 2011. Experimental characterization of West African Newcastle disease virus [abstract]. RESOLAB Annual Directors Meeting. p. 1.

Characterization of Newcastle disease virus isolated from cormorant and gull species in the United States in 2010 - (Peer Reviewed Journal)

Diel, D.G., Miller, P.J., Wolf, P.C., Mickley, R.M., Musante, A.R., Emanueli, D.C., Shively, K.J., Afonso, C.L. 2012. Characterization of Newcastle disease virus isolated from cormorant and gull species in the United States in 2010. Avian Diseases. 56:128-133.

Complete genome and clinic opathological characterization of a virulent Newcastle disease virus isolate from South America - (Peer Reviewed Journal)

Diel, D.G., Susta, L., Cardenas, S., Brown, C.C., Miller, P.J., Afonso, C.L. 2012. Complete genome and clinic opathological characterization of a virulent Newcastle disease virus isolate from South America. Journal of Clinical Microbiology. 50:378-387.

Comparative full genome analysis of four infectious laryngotracheitis virus (gallid herpesvirus-1) virulent isolates from the United States - (Peer Reviewed Journal)

Spatz, S.J., Keeler, C.L., Kutish, G.F., Riblet, S.M., Volkening, J.D., Zsak, L., Afonso, C.L., Mundt, E.S., Rock, D.L., Garcia, M. 2012. Comparative full genome analysis of four infectious laryngotracheitis virus (gallid herpesvirus-1) virulent isolates from the United States. Virus Genes. 44:273-85.

Exchange of Newcastle disease virus fusion and hemagglutinin-neuraminidase genes into a vaccine backbone: effects on virulence - (Abstract Only)

Cardenas Garcia, S., Susta, L., Diel, D.G., Decanini, E.L., Absalon, A., Brown, C., Yu, Q., Miller, P.J., Afonso, C.L. 2012. Exchange of Newcastle disease virus fusion and hemagglutinin-neuraminidase genes into a vaccine backbone: effects on virulence . Meeting Abstract. 50:378-387.

Complete genome sequence and clinicopathological characterization of a virulent Newcastle disease virus isolated from poultry in South America - (Abstract Only)

Diel, D.G., Susta, L., Cardenas Garcia, S., Killian, M., Brown, C., Miller, P.J., Afonso, C.L. 2012. Complete genome sequence and clinicopathological characterization of a virulent Newcastle disease virus isolated from poultry in South America. Meeting Abstract. Vol.50, pages 378-387.

Bioinformatic analysis of variability of Newcastle disease virus diagnostic primers and probes and the potential for false negative detection - (Abstract Only)

Suarez, D.L., Afonso, C.L. 2011. Bioinformatic analysis of variability of Newcastle disease virus diagnostic primers and probes and the potential for false negative detection [abstract]. 1st International Respiratory Disease Converence, May 15-18, 2011, Athens, Georgia. p. 13.

Newcastle disease virus - (Review Article)

Miller, P.J., Afonso, C.L. 2011. Newcastle disease virus. Encyclopedia of Life Sciences. In: eLS, 2010, John Wiley & Sons, Ltd: Chichester. Available: http://www.els.net. DOI: 10.1002/9780470015902.a0001077.pub3.

Neurovirulent pathotype of Newcastle disease virus associated with the reduced capacity of NDV to replicate in vivo and in vitro - (Abstract Only)

Susta, L., Miller, P.J., Hu, S., Liu, X., Rue, C.A., Afonso, C.L., Brown, C.C. 2011. Neurovirulent pathotype of Newcastle disease virus associated with the reduced capacity of NDV to replicate in vivo and in vitro [abstract]. 1st International Avian Respiratory Disease Converence, May 15-18, 2011, Athens, Georgia. p. 58.

The effect of IL-2 expression by recombinant Newcastle disease virus on host immune response, viral replication and pathogenesis - (Abstract Only)

Edwards, I.C., Susta, L., Cardenas, S., Miller, P.J., Brown, C.C., Afonso, C.L. 2011. The effect of IL-2 expression by recombinant Newcastle disease virus on host immune response, viral replication and pathogenesis [abstract]. 1st International Avian Respiratory Disease Conference, May 15-18, 2011, Athens, Georgia. p. 34.

Construction of recombinant Newcastle disease viruses expessing chicken IFN-gamma: effect of elevated levels of expression on the transcriptional host response, viral replication and pathogenesis - (Abstract Only)

Susta, L., Edwards, I.C., Cardenas, S., Miller, P.J., Brown, C.C., Afonso, C.L. 2011. Construction of recombinant Newcastle disease viruses expessing chicken IFN-gamma: effect of elevated levels of expression on the transcriptional host response, viral replication and pathogenesis [abstract]. 1st International Avian Respiratory Disease Conference, May 15-18, 2011, Athens, Georgia. p. 15.

Virulent Newcastle disease viruses isolated from cormorant and gull species in the states of the Atlantic Flyway in 2010 - (Abstract Only)

Miller, P.J., Wolf, P.C., Mickley, R.M., Mustante, A.R., Emanueli, D.C., Shively, K.J., Afonso, C.L. 2011. Virulent Newcastle disease viruses isolated from cormorant and gull species in the states of the Atlantic Flyway in 2010 [abstract]. 1st International Avian Respiratory Disease Conference, May 15-18, 2011, Athens, Georgia. p. 54.

In vivo transcriptional cytokine responses and association with clinical and pathological outcomes in chickens infected with different Newcastle disease virus isolates using formalin-fixed paraffin-embedded samples - (Peer Reviewed Journal)

Ecco, R., Brown, C.C., Susta, L., Cagle, C.A., Edwards, I.C., Pantin Jackwood, M.J., Miller, P.J., Afonso, C.L. 2011. In vivo transcriptional cytokine responses and association with clinical and pathological outcomes in chickens infected with different Newcastle disease virus isolates using formalin-fixed paraffin-embedded samples. Veterinary Immunology and Immunopathology. 141:221-229.

Early occurrence of apoptosis in lymphoid tissues from chickens infected with strains of Newcastle disease virus of varying virulence - (Peer Reviewed Journal)

Harrison, L., Brown, C.C., Afonso, C.L., Zhang, J., Susta, L. 2011. Early occurrence of apoptosis in lymphoid tissues from chickens infected with strains of Newcastle disease virus of varying virulence. Journal of Comparative Pathology. 145:327-335.

Avian paramyxovirus serotype 1 strains of low virulence with unusual fusion protein cleavage sites isolated from poultry species - (Abstract Only)

Miller, P.J., Killian, M.L., Pederson, J.C., Afonso, C.L. 2011. Avian paramyxovirus serotype 1 strains of low virulence with unusual fusion protein cleavage sites isolated from poultry species [abstract]. American Association of Avian Pathologists Annual Meeting, July 16-19, 2011, St. Louis, Missouri. p. 114.

Analysis of transcriptional responses of chickens infected with different Newcastle disease virus isolates using paraffin embedded samples - (Abstract Only)

Ecco, R., Brown, C.C., Edwards, I.C., Cagle, C.A., Miller, P.J., Susta, L., Pantin Jackwood, M.J., Afonso, C.L. 2011. Analysis of transcriptional responses of chickens infected with different Newcastle disease virus isolates using paraffin embedded samples [abstract]. American Association of Avian Pathologists Annual Meeting, July 16-19, 2011, St. Louis, Missouri. p. 14.

Virulent Newcastle disease virus elicits a strong innate immune response in chickens - (Peer Reviewed Journal)

Rue, C.A., Susta, L., Edwards, I.C., Brown, C.C., Kapczynski, D.R., Suarez, D.L., King, D.J., Miller, P.J., Afonso, C.L. 2011. Virulent Newcastle disease virus elicits a strong innate immune response in chickens. Journal of General Virology. 92:931-939.

Update on poultry viral diseases research conducted at Southeast Poultry Research Laboratory - (Abstract Only)

Pantin Jackwood, M.J., Swayne, D.E., Spackman, E., Suarez, D.L., Afonso, C.L., Miller, P.J., Zsak, L., Day, J.M., Yu, Q. 2011. Update on poultry viral diseases research conducted at Southeast Poultry Research Laboratory [abstract]. Proceedings of the United States Animal Health Association 114th Annual Meeting, November 17, 2010, Minneapolis, MN. p. 526-527.

Neurological lesions in chickens experimentally infected with virulent Newcastle disease virus isolates - (Peer Reviewed Journal)

Ecco, R., Susta, L., Afonso, C.L., Miller, P.J., Brown, C.C. 2011. Neurological lesions in chickens experimentally infected with virulent Newcastle disease virus isolates. Avian Pathology. 40(2):145-152.

Comparative nucleotide sequence analysis of three virulent strains of infectious laryngotracheitis virus - (Proceedings)

Spatz, S.J., Afonso, C.L., Zsak, L., Garcia, M., Mundt, E., Riblet, S., Keeler, C., Rock, D. 2010. Comparative nucleotide sequence analysis of three virulent strains of infectious laryngotracheitis virus. Workshop on Molecular Pathogenesis of Marek's Disease and Avian Immunology. 1(1): 59.

Evidence for a new avian paramyxovirus serotype-10 detected in Rockhopper penguins from the Falkland Islands - (Peer Reviewed Journal)

Miller, P.J., Afonso, C.L., Spackman, E., Scott, M.A., Pedersen, J.C., Senne, D.A., Brown, J.D., Fuller, C.M., Uhart, M.M., Karesh, W.B., Brown, I.H., Alexander, D.J., Swayne, D.E. 2010. Evidence for a new avian paramyxovirus serotype-10 detected in Rockhopper penguins from the Falkland Islands. Journal of Virology. 84(21):11496-11504.

Comparative nucleotide sequence analysis of three virulent strains of infectious laryngotracheitis virus (ILTV) - (Proceedings) - (21-Jun-10)

Pathogenicity evaluation of different Newcastle disease virus chimeras in 4-week-old chickens - (Peer Reviewed Journal)

Susta, L., Miller, P.J., Afonso, C.L., Estevez, C., Yu, Q., Zhang, J., Brown, C.C. 2010. Pathogenicity evaluation of different Newcastle disease virus chimeras in 4-week-old chickens. Tropical Animal Health and Production. 42(8):1785-1795.

Clinicopathological characterization in poultry of three strains of Newcastle disease viruses isolated from recent outbreaks - (Peer Reviewed Journal)

Susta, L., Miller, P.J., Afonso, C.L., Brown, C.C. 2011. Clinicopathological characterization in poultry of three strains of Newcastle disease viruses isolated from recent outbreaks. Veterinary Pathology. 48(2):349-360.

Phylogenetic and pathological characterization of Newcastle disease virus isolates from Pakistan - (Peer Reviewed Journal)

Khan, T.A., Rue, C.A., Rehmani, S.F., Ahmed, A., Wasilenko, J.L., Miller, P.J., Afonso, C.L. 2010. Phylogenetic and pathological characterization of Newcastle disease virus isolates from Pakistan. Journal of Clinical Microbiology. 48(5):1892-1894.

Neurological lesions in chickens experimentally infected with virulent Newcastle disease virus isolates - (Abstract Only)

Ecco, R., Susta, L., Afonso, C.L., Miller, P.J., Brown, C.C. 2010. Neurological lesions in chickens experimentally infected with virulent Newcastle disease virus isolates [abstract]. In: Proceedings of the American Association of Avian Pathologists annual meeting, July 31-August 04, 2010, Atlanta, Georgia. Paper #9393.

Evolutionary changes effecting rapid diagnostics of 2009 Newcastle disease viruses isolated from Double-crested Cormorants - (Peer Reviewed Journal)

Rue, C.A., Susta, L., Brown, C.C., Pasick, J.M., Swafford, S.R., Wolf, P.C., Killian, M.L., Pedersen, J.C., Miller, P.J., Afonso, C.L. 2010. Evolutionary changes effecting rapid diagnostics of 2009 Newcastle disease viruses isolated from Double-crested Cormorants. Journal of Clinical Microbiology. 48(7):2440-2448.

Presence of apoptosis, as determined by immunohistochemistry, in lymphoid tissues of chickens infected with strains of Newcastle disease virus of varying virulence - (Abstract Only)

Susta, L., Harrison, L., Zhang, J., Afonso, C.L., Miller, P.J., Brown, C.C. 2009. Presence of apoptosis, as determined by immunohistochemistry, in lymphoid tissues of chickens infected with strains of Newcastle disease virus of varying virulence [abstract]. American Association of Avian Pathologists/American Veterinary Medical Association Annual Meeting, July 11-15, 2009, Seattle, Washington. p. 16.

Pathological and immunohistochemical study of the reproductive tracts of vaccinated layers infected with the virulent CA02 strain of Newcastle disease virus - (Abstract Only)

Susta, L., Miller, P.J., Decanini, E.L., Absalon, A.E., Afonso, C.L., Brown, C.C. 2010. Pathological and immunohistochemical study of the reproductive tracts of vaccinated layers infected with the virulent CA02 strain of Newcastle disease virus [abstract]. In: Proceedings of the American Association of Avian Pathologists annual meeting, July 31-August 04, 2010, Atlanta, Georgia. Paper #9394.

Levels of humoral antibodies induced by different inactivated vaccines correlate with egg production in commercial layers challenged with virulent Newcastle disease virus - (Abstract Only)

Miller, P.J., Susta, L., Brown, C.C., Decanini, E.L., Absalon, A.E., Afonso, C.L. 2010. Levels of humoral antibodies induced by different inactivated vaccines correlate with egg production in commercial layers challenged with virulent Newcastle disease virus [abstract]. In: Proceedings of the American Association of Avian Pathologists Annual Meeting, July 31-August 04, 2010, Atlanta, Georgia. paper #9300.

Clinicopathological characterization of two recombinant Newcastle disease viruses derived from a virulent Chinese strain - (Abstract Only)

Susta, L., Miller, P.J., Hu, S., Liu, X., Rue, C.A., Afonso, C.L., Brown, C.C. 2010. Clinicopathological characterization of two recombinant Newcastle disease viruses derived from a virulent Chinese strain [abstract}. In: Proceedings of the American Association of Avian Pathologists annual meeting, July 31-August 04, 2010, Atlanta, Georgia. Paper #9392.

Newcastle disease: New challenges in diagnostic and vaccination - (Abstract Only)

Pantin Jackwood, M.J., Afonso, C.L., Miller, P.J. 2009. Newcastle disease: New challenges in diagnostic and vaccination [abstract]. 44th United States-Japan Cooperative Program in Natural Resources, November 30-December 5, 2009, Tsukuba, Japan. p. 33.

Research update from Southeast Poultry Research Laboratory - (Abstract Only)

Suarez, D.L., Swayne, D.E., Pantin Jackwood, M.J., Afonso, C.L., Miller, P.J., Kapczynski, D.R. 2010. Research update from Southeast Poultry Research Laboratory [abstract]. Report of the 113th United States Animal Health Association meeting, October 13, 2009, San Diego, California. p. 26-27.

Newcastle disease: Evolution of genotypes and the related diagnostic challenges - (Peer Reviewed Journal)

Miller, P.J., Decanini, E.L., Afonso, C.L. 2010. Newcastle disease: Evolution of genotypes and the related diagnostic challenges. Infection, Genetics and Evolution. 10(1):26-35.

Evolutionary dynamics of Newcastle disease virus - (Peer Reviewed Journal)

Miller, P.J., Kim, L.M., Afonso, C.L., Ip, H.S. 2009. Evolutionary dynamics of Newcastle disease virus. Virology. 391:64-72.

Pathogenesis study of selected velogenic strains of Newcastle disease virus in White Leghorn chickens - (Abstract Only)

Susta, L., Miller, P.J., Hu, S., Liu, Z., Rue, C.A., Afonso, C.L., Brown, C.C. 2009. Pathogenesis study of selected velogenic strains of Newcastle disease virus in White Leghorn chickens [abstract]. In: Proceedings of the 14th International Symposium for the World Association of Veterinary Laboratory Diagnosticians, WAVLD 2009, June 17-20, 2009, Madrid, Spain. p. 222.

Role of immune-related host gene responses in the pathobiology of H5N1 highly pathogenic avian influenza in ducks - (Abstract Only)

Pantin Jackwood, M.J., Kapczynski, D.R., Sarmento, L., Wasilenko, J.L., Afonso, C.L. 2009. Role of immune-related host gene responses in the pathobiology of H5N1 highly pathogenic avian influenza in ducks [abstract]. 7th International Symposium on Avian Influenza, April 5-9, 2009, Athens, Georgia. p. 43.

A highly virulent strain of Newcastle disease virus elicits a strong innate response and nitric oxide production in chickens - (Abstract Only)

Rue, C.A., Susta, L., Brown, C.C., Kapczynski, D.R., Miller, P.J., King, D.J., Suarez, D.L., Afonso, C.L. 2009. A highly virulent strain of Newcastle disease virus elicits a strong innate response and nitric oxide production in chickens [abstract]. American Association of Avian Pathologists Annual Meeting, July 11-14, 2009, Seattle, Washington. p. 16.

Transmission of virulent Newcastle disease virus (NDV) between unvaccinated, sub-optimally vaccinated, and well-vaccinated SPF chickens - (Abstract Only)

Miller, P.J., Afonso, C.L. 2009. Transmission of virulent Newcastle disease virus (NDV) between unvaccinated, sub-optimally vaccinated, and well-vaccinated SPF chickens [abstract]. American Association of Avian Pathologists/American Veterinary Medical Association annual meeting, July 11-15, 2009, Seattle, Washington. Paper #7606.

Use of genomic interspecies microarray hybridization to detect differentially expressed genes associated with H5N1 avian influenza virus infections in ducks - (Abstract Only)

Pantin Jackwood, M.J., Sarmento, L., Afonso, C.L. 2008. Use of genomic interspecies microarray hybridization to detect differentially expressed genes associated with H5N1 avian influenza virus infections in ducks [abstract]. Proceedings of the American Association of Veterinary Laboratory Diagnosticians Annual Meeting, October 23-29, 2008, Greensboro, NC. 2008 CDROM.

Biological and phylogenetic characterization of pigeon paramyxovirus serotype-1 circulating in North American pigeons - (Peer Reviewed Journal)

Kim, L.M., King, D.J., Guzman, H., Tesh, R.B., Bueno, R., Dennett, J.A., Afonso, C.L. 2008. Biological and phylogenetic characterization of pigeon paramyxovirus serotype-1 circulating in North American pigeons and doves. Journal of Clinical Microbiology. 46(10):3303-3310.

Not so fast on recombination analysis of Newcastle disease virus - (Other)

Afonso, C.L. 2008. Not so fast on recombination analysis of Newcastle disease virus. Journal of Virology. 82(18):9303.

Full genome sequencing of the Newcastle disease viruses VS/GA and clone 5 - (Peer Reviewed Journal)

Perozo, F., Villegas, P., Afonso, C.L. 2008. Genomic comparison of the complete coding and intergenic regions of the VG/GA Newcastle disease virus and its respirotropic clone 5. Virus Genes. 37(2):161-167.

Highly pathogenic H5N1 avian influenza viruses differentially affect gene expression in primary chicken embryo fibroblasts - (Peer Reviewed Journal)

Sarmento, L., Afonso, C.L., Estevez, C., Wasilenko, J.L., Pantin Jackwood, M.J. 2008. Differential host gene expression in cells infected with highly pathogenic H5N1 avian influenza viruses. Veterinary Immunology and Immunopathology. 125:291-302.

Biological and phylogenic characterization of virulent Newcastle disease virus circulating in Mexico - (Peer Reviewed Journal)

Perozo, F., Merino, R., Afonso, C.L., Villegas, P., Calderon, N. 2008. Biological and phylogenic characterization of virulent Newcastle disease virus circulating in Mexico. Avian Diseases. 52(3):472-479.

Microarray analysis following infection with highly pathogenic avian influenza H5N1 virus in naive and vaccinated SPF chickens - (Abstract Only)

Kapczynski, D.R., Afonso, C.L., Pantin Jackwood, M.J., Liljebjelke, K.A., Sarmento, L., Suarez, D.L., Swayne, D.E. 2008. Microarray analysis following infection with highly pathogenic avian influenza H5N1 virus in naive and vaccinated SPF chickens [abstract]. In: Proceedings of Avian Immunology Research Group Conference, June 24-27, 2008, Queensland, Australia. p. 22.

Detection of a broad range of class I and II Newcastle disease viruses using a multiplex real time RT-PCR assay - (Peer Reviewed Journal)

Kim, L.M., Suarez, D.L., Afonso, C.L. 2008. Detection of a broad range of class I and II Newcastle disease viruses using a multiplex real-time reverse transcription polymerase chain reaction assay. Journal of Veterinary Diagnostic Investigation. 20:414-425.

The VG/GA strain of Newcastle disease virus: Mucosal immunity, protection against lethal challenge and molecular analysis - (Peer Reviewed Journal)

Perozo, F., Villegas, P., Dolz, R., Afonso, C.L., Purvis, L.B. 2008. The VG/GA strain of Newcastle disease virus: Mucosal immunity, protection against lethal challenge and molecular analysis. Avian Pathology. 37(3):237-245.

Phylogenetic and biological characterization of virulent Newcastle disease viruses isolated in wild birds during 2002-2007 - (Abstract Only)

Afonso, C.L., King, D.J., Kim, L.M., Guzman, H., Tesh, R.B., Dennett, J.A. 2000. Phylogenetic and biological characterization of virulent Newcastle disease viruses isolated in wild birds during 2002-2007 [abstract]. In: Proceedings of the American Association of Avian Pathologists meeting, July 19-23, 2008, New Orleans, Louisiana. p. 118.

Gene expression responses to highly pathogenic avian influenza H5N1 virus infections in ducks - (Abstract Only)

Pantin Jackwood, M.J., Kapczynski, D.R., Wasilenko, J.L., Sarmento, L., Afonso, C.L. 2008. Gene expression responses to highly pathogenic avian influenza H5N1 virus infections in ducks [abstract]. 145th American Medical Association Annual Meeting, July 20-23, 2008, New Orleans, Louisiana. 2008 CDROM.

Viral genome sequencing bt random priming methods - (Peer Reviewed Journal)

Djikeng, A., Halpin, R., Kuzmickas, R., Sengamalay, N., Afonso, C.L., Xang, X., Anderson, N.G., Ghedin, E., Spiro, D.J. 2008. Viral genome sequencing by random priming methods. Biomed Central (BMC) Genomics. 9:5.

Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian invluenza virus - (Peer Reviewed Journal)

Sarmento, L., Pantin Jackwood, M.J., Kapczynski, D.R., Swayne, D.E., Afonso, C.L. 2008. Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian invluenza virus. Biologicals. 132:175-183.

Baculoviruses: Molecular Biology of Mosquito Baculoviruses - (Peer Reviewed Journal)

Becnel, J.J., Afonso, C.L. 2008. Baculoviruses: Molecular Biology of Mosquito Baculoviruses. Encyclopedia of virology, 5 vols. (B.W.J. Mahy and M.H.V. Van Regenmortel, Editors), pp. 219-225 Oxford:Elsevier.

Characterization and phylogenic analysis of Mexican Newcastle disease virus isolates - (Abstract Only)

Merino, R., Calderon, N., Perozo, F., Villegas, P., Afonso, C.L. 2008. Characterization and phylogenic analysis of Mexican Newcastle disease virus isolates [abstract]. International Poultry Scientific Forum, January 21-22, 2008, Atlanta, Georgia. p. 33.

Variable regulation of chicken embryo fibroblasts gene expression by H5N1 avian influenza viruses - (Abstract Only)

Sarmento, L., Pantin Jackwood, M.J., Afonso, C.L., Wasilenko, J.L., Estevez, C. 2008. Variable regulation of chicken embryo fibroblasts gene expression by H5N1 avian influenza viruses [abstract]. International Poultry Scientific Forum, January 21-22, 2008, Atlanta, Georgia. p. 33.

Primer sequence-independent full genome amplification and sequencing of the VG/GA strain of Newcastle disease virus - (Abstract Only)

Perozo, F., Villegas, P., Afonso, C.L. 2008. Primer sequence-independent full genome amplification and sequencing of the VG/GA strain of Newcastle disease virus [abstract]. International Poultry Scientific Forum, January 21-22, 2008, Atlanta, Georgia. p. 32.

Research update on exotic and emerging poultry diseases - (Proceedings)

Pantin Jackwood, M.J., Suarez, D.L., Zsak, L., Spackman, E., Kapczynski, D.R., King, D.J., Afonso, C.L., Day, J.M., Spatz, S.J., Yu, Q., Swayne, D.E. 2007. Research update on exotic and emerging poultry diseases. In: Proceedings of the 111th Annual Meeting of the U. S. Animal Health Association, Reno, Nevada, October 22-23, 2007. p. 702-707. Available: http://www.usaha.org/meetings/2007/2007_USAHA_Proceedings.pdf.

A primary chicken tracheal cell culture system for the study of infection with avian respiratory viruses - (Peer Reviewed Journal)

Zaffuto, K.M., Estevez, C., Afonso, C.L. 2008. Primary chicken tracheal cell culture system for the study of infection with avian respiratory viruses. Avian Pathology. 37(1):25-31.

Phylogenetic diversity among low virulence Newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to poultry-origin isolates - (Peer Reviewed Journal)

Kim, L.M., King, D.J., Curry, P., Suarez, D.L., Swayne, D.E., Stallknecht, D., Slemons, R.D., Pedersen, J.C., Senne, D.S., Winker, K., Afonso, C.L. 2007. Phylogenetic diversity among low-virulence Newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to those of poultry-origin isolates. Journal of Virology. 81(22):12641-12653.

Sheeppox virus kelch-like gene SPPV-019 affects virus virulence - (Peer Reviewed Journal)

Balinsky, C.A., Delhon, G., Afonso, C.L., Risatti, G.R., Borca, M.V., French, R.A., Tulman, E.R., Geary, S.J., Rock, D.L. 2007. Sheeppox virus kelch-like gene SPPV-019 affects virus virulence. Journal of Virology. 81(20):11392-11401.

Antigenic differences among NDV strains of different genotypes used in vaccine formulation affects viral shedding after a virulent challenge - (Peer Reviewed Journal)

Miller, P.J., King, D.J., Afonso, C.L., Suarez, D.L. 2007. Antigenic differences among NDV strains of different genotypes used in vaccine formulation affects viral shedding after a virulent challenge. Vaccine. 25:7238-7246.

Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages - (Peer Reviewed Journal)

Zaffuto, K.M., Piccone, M.E., Burrage, T.G., Risatti, G.R., Borca, M.V., Holinka, L.G., Rock, D.L., Afonso, C.L. 2007. Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages. Journal of General Virology. 88:3007-3012.

Early responses of chicken lungs and spleens to infection with highly pathogenic avian influenza virus using microarray analysis - (Abstract Only)

Afonso, C.L., Kapczynski, D.R., Pantin Jackwood, M.J., Sarmento, L., Swayne, D.E. 2007. Early responses of chicken lungs and spleens to infection with highly pathogenic avian influenza virus using microarray analysis [abstract]. In: Proceedings of the International Symposium on Animal Genomics for Animal Health, October 23-25, 2007, Paris France. p. 59.

Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian influenza - (Abstract Only)

Afonso, C.L., Sarmento, L., Pantin Jackwood, M.J., Kapczynski, D.R., Swayne, D.E. 2007. Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian influenza [abstract]. In: Proceedings of the International Symposium on Animal Genomics for Animal Health, October 23-25, 2007, Paris, France. p. 16.

Detection and isolation of exotic Newcastle disease (ENDV) from field collected flies - (Peer Reviewed Journal)

Chakrabarti, S., King, D.J., Afonso, C.L., Swayne, D.E., Cardona, C.J., Kuney, D.R., Gerry, A.C. 2007. Detection and isolation of exotic Newcastle disease virus from field-collected flies. Journal of Medical Entomology. 44(5):840-844.

Design of a multiplex real time RT-PCR assay to detect Newcastle disease viruses from classes I and II - (Abstract Only)

Kim, L.M., Suarez, D.L., Afonso, C.L. 2007. Design of a multiplex real time RT-PCR assay to detect Newcastle disease viruses from classes I and II [abstract]. 10th International Conference for Biodetection Technologies 2007, June 14-15, 2007, Atlanta, Georgia. Poster #8.

Sequencing of avian influenza virus genomes following random amplification - (Peer Reviewed Journal)

Afonso, C.L. 2007. Sequencing of avian influenza virus genomes following random amplification. Biotechniques. 43(2):188-192.

Isolation and genetic characterization of influenza A viruses and a paramyxovirus from wild birds in Barbados: 2003-2004 - (Peer Reviewed Journal)

Douglas, K.O., Lavoie, M.L., Suarez, D.L., Afonso, C.L., Kim, L.M. 2007. Isolation and genetic characterization of avian influenza viruses and a Newcastle disease virus from wild birds in Barbados: 2003-2004. Avian Diseases. 51(3):781-787.

Isolation of exotic Newcastle disease virus (ENDV) from field collected flies and experimental ENDV infections of three arthropod species - (Abstract Only)

King, D.J., Chakrabarti, S., Cardona, C., Afonso, C.L., Swayne, D.E., Gerry, A.C. 2007. Isolation of exotic Newcastle disease virus (ENDV) from field collected flies and experimental ENDV infections of three arthropod species [abstract]. American Association of Avian Pathologists/American Veterinary Medical Association Meeting Program, July 14-18, 2007, Washington, DC. p. 44.

Characterization of Class I Newcastle disease virus isolates from Hong Kong bird markets and detection using real-time reverse transcription PCR - (Peer Reviewed Journal)

Kim, L.M., King, D.J., Suarez, D.L., Wong, C.W., Afonso, C.L. 2007. Characterization of Class I Newcastle disease virus isolates from Hong Kong bird markets and detection using real-time reverse transcription PCR. Journal of Clinical Microbiology. 45:1310-1314.

Newcastle disease virus isolates from US waterfowl reveal novel genomic subgroups with diverse and evolving genetics and the potential for spillover into US live bird markets - (Abstract Only)

Kim, L.M., King, D.J., Swayne, D.E., Stallknecht, D., Slemons, R.D., Pedersen, J.C., Senne, D.A., Winker, K., Afonso, C.L. 2007. Newcastle disease virus isolates from US waterfowl reveal novel genomic subgroups with diverse and evolving genetics and the potential for spillover into US live bird markets [abstract]. In: Abstracts of the American Association of Avian Pathologists meeting, July 14-19, 2007, Washington, DC. p. 43. CD.

Differential growth of avian influenza virus in chicken and duck cells - (Abstract Only)

Sarmento, L., Zaffuto, K.M., Pantin Jackwood, M.J., Afonso, C.L. 2007. Differential growth of avian influenza virus in chicken and duck cells [abstract]. 144th American Veterinary Medical Association Annual Meeting, July 14-19, 2007, Washington, DC. CD-ROM.

Development and characterization of a primary culture of chicken embryonic tracheal epithelial cells and their use in avian studies - (Abstract Only)

Zaffuto, K.M., Suarez, D.L., Estevez, C., Afonso, C.L. 2007. Development and characterization of a primary culture of chicken embryonic tracheal epithelial cells and their use in avian studies [abstract]. American Association of Avian Pathologists Annual Meeting, July 14-19, 2007, Washington, DC. p. 17.

Why are avian influenza viruses emerging and what tools are needed to prevent and control the infection and disease? - (Abstract Only)

Swayne, D.E., Suarez, D.L., Pantin Jackwood, M.J., Spackman, E., Kapczynski, D.R., Afonso, C.L. 2007. Why are avian influenza viruses emerging and what tools are needed to prevent and control the infection and disease?. In: Proceedings of the 110th Annual Meeting of the U.S. Animal Health Association, October 12-18, 2006, Minneapolis, Minnesota. p. 728-729.

Use of bioinformatics in improving detection of Newcastle disease virus - (Book / Chapter)

Kim, L.M., Suarez, D.L., Afonso, C.L. 2007. Use of bioinformatics in improving detection of Newcastle disease virus. In: Rogeria de Almeida, M., Pires Moraes, M., Patarroyo, J.H., Vidigal, P.M.P., Borem, A., editors. Biotecnologia e Saude Animal. Viscoza, Brazil: Federal University of Viscosa. Chapter 9. p. 227-249.

Genus Suipoxvirus - (Book / Chapter)

Gustavo, D.A., Tulman, E.R., Afonso, C.L., Rock, D.L. 2007. Genus Suipoxvirus. In: Mercer, A., Schmidt, A., Weber, O. (editors). Poxviruses. Birkhauser Basel. p. 203-215.

RECOVERY OF EXOTIC NEWCASTLE DISEASE VIRUS (ENDV) FROM FIELD COLLECTED FLIES AND EXPERIMENTAL ENDV INFECTIONS OF THREE ARTHROPOD SPECIES - (Abstract Only)

Chakrabarti, S., King, D.J., Cardona, C., Afonso, C.L., Swayne, D.E., Gerry, A.C. 2006. Recovery of exotic Newcastle disease virus (ENDV) from field collected flies and experimental ENDV infections of three arthropod species [abstract]. The 54th Annual Meeting of the Entomological Society of America, December 10-13, 2006, Indianapolis, Indiana. p. 93.

GENOME OF INVERTEBRATE IRIDESCENT VIRUS 3 (MOSQUITO IRIDESCENT VIRUS) - (Peer Reviewed Journal)

Delhon, G., Tulman, E., Afonso, C.L., Lu, Z., Becnel, J.J., Moser, B.A., Kutish, G.F., Rock, D.L. 2006. Genome of invertebrate iridescent virus 3 (mosquito iridescent virus). Journal of Virology. 80(17):8439-8449.

GENOME OF HORSEPOX VIRUS - (Peer Reviewed Journal)

Tulman, E., Delhon, G., Afonso, C.L., Lu, Z., Zsak, L., Sandybaev, N.T., Kerembekova, U.Z., Zaitsev, V.L., Kutish, G.F., Rock, D.L. 2006. Genome of horsepox virus. Journal of Virology. 80(18):9244-9258.

POTENTIAL ROLE OF FLIES IN THE PERSISTENCE AND DISPERSAL OF EXOTIC NEWCASTLE DISEASE VIRUS IN SOUTHERN CALIFORNIA - (Abstract Only)

Chakrabarti, S., King, D.J., Cardona, C., Afonso, C.L., Swayne, D.E., Gerry, A.C. 2006. Potential role of flies in the persistence and dispersal of exotic Newcastle disease virus in Southern California [abstract]. Livestock Insect Worker's Conference Annual Meeting. p. 34.

PHYLOGENETIC CHARACTERIZATION OF ENDEMIC NEWCASTLE DISEASE VIRUSES ISOLATED FROM WILD BIRDS DURING 2000 TO 2004 IN DELAWARE, MARYLAND AND NEW JERSEY - (Abstract Only)

Afonso, C.L. 2006. Phylogenetic characterization of endemic Newcastle disease viruses isolated from wild birds during 2000 to 2004 in Delaware, Maryland and New Jersey [abstract]. American Association of Avian Pathologists 2006 Meeting, Hawaii. p. 154.

EFFECT OF PROBE-SITE MISMATCHES ON DETECTION OF VIRULENT NEWCASTLE DISEASE VIRUSES USING A FUSION-GENE REAL-TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION TEST - (Peer Reviewed Journal)

Kim, L.M., Afonso, C.L., Suarez, D.L. 2006. Effect of probe-site mismatches on detection of virulent Newcastle disease viruses using a fusion-gene real-time reverse transcription polymerase chain reaction test. Journal of Veterinary Diagnostic Investigation. 18:519-528.

REAL TIME RT-PCR DEVELOPED FOR A PHYLOGENETICALLY DIVERGENT GROUP OF NEWCASTLE VIRUSES NOT DETECTED BY CURRENT TESTS - (Abstract Only)

Kim, L.M., Afonso, C.L., Suarez, D.L., King, D.J. 2006. Real time RT-PCR developed for a phylogenetically divergent group of Newcastle viruses not detected by current tests [abstract]. American Association of Avian Pathologists. CD p. 156.

GENOME OF CROCODILEPOX VIRUS - (Peer Reviewed Journal)

Afonso, C.L., Tulman, E.R., Delhon, G., Lu, Z., Viljoen, G.J., Wallace, D.B., Kutish, G.F., Rock, D.L. 2006. Genome of crocodilepox virus. Journal of Virology. 80(10)4978-4991.

CONTRASTING RESULTS FROM MOLECULAR AND BIOLOGICAL PATHOGENICITY ASSAYS OF NEWCASTLE DISEASE VIRUS ISOLATE IOWA 1519 - (Abstract Only)

King, D.J., Kapczynski, D.R., Afonso, C.L., Piacenti, A. 2005. Contrasting results from molecular and biological pathogenicity assays of newcastle disease virus isolate Iowa 1519 [abstract]. American Association of Avian Pathologists, p.33.

GENOME SEQUENCING AND ANALYSIS - (Proceedings)

Afonso, C.L., Kutish, G.F. 2004. Genome sequencing and analysis [abstract]. Society of Invertebrate Pathology.

AFRICAN SWINE FEVER VIRUS MULTIGENE FAMILY 360 AND 530 GENES AFFECT HOST INTERFERON RESPONSE - (Peer Reviewed Journal)

Afonso, C., Piccone, M.E., Zaffuto, K.M., Neilan, J.G., Kutish, G., Lu, Z., Balinsky, C.A., Gibb, T., Bean, T.R., Zsak, L., Rock, D.L. 2004. African swine fever virus multigene family 360 and 530 genes affect host interferon response.. Journal of Virology. (2004) 78 (4):1858-1864.

AFRICAN SWINE FEVER VIRUS MULTIGENE FAMILY 360 AND 530 GENES AFFECT HOST INTERFERON RESPONSE - (Peer Reviewed Journal)

Afonso, C.L., Piccone, M.E., Zaffuto, K., Neilan, J.G., Kutish, G.F., Lu, Z., Balinsky, C.A., Gibb, T.R., Zsak, L., Rock, D.L. 2004. African swine fever virus multigene family 360 and 530 genes affect host interferon response. Journal of Virology. 78(4):1858-1864.

GENOMES OF PARAPOXVIRUSES ORF VIRUS AND BOVINE PAPULAR STOMATITIS VIRUS - (Peer Reviewed Journal)

Delhon, G., Tulman, E., Afonso, C.L., Lu, Z., Concha-Bermejillo, A., Lehmkuhl, H., Piccone, M.E., Kutish, G.F., Rock, D.L. 2004. Genomes of parapoxviruses orf virus and bovine papular stomatitis virus. Journal of Virology. 78(1):168-177.

GENOMES OF THE PARAPOXVIRUSES ORF VIRUS AND BOVINE PAPULAR STOMATITIS VIRUS - (Peer Reviewed Journal)

Delhon, G., Tulman, E., Afonso, C., Lu, Z., De La Concha-Bermeji, A., Lehmkuhl, H., Piccone, M.E., Kutish, G., Rock, D.L. 2004. Genomes of the parapoxviruses orf virus and bovine papular stomatitis virus.. Journal of Virology. 78(1):168-177.

THE GENOME OF CANARYPOX VIRUS - (Peer Reviewed Journal)

Tulman, E., Afonso, C.L., Lu, Z., Zsak, L., Kutish, G.F., Rock, D.L. 2004. The genome of canarypox virus. Journal of Virology. 78(1):353-366.

HZ-2V GENOME ANALYSIS - (Proceedings)

Kim, W., Burand, J.P., Afonso, C.L., Kutish, G.F., Lu, Z., Rock, D.L. 2003.Hz-2v genome analysis [abstract]. Society for Invertebrate Pathology Annual Meeting. P. 78.

GENOME OF BOVINE HERPESVIRUS 5 - (Peer Reviewed Journal)

Delhon, G., Moraes, M.P., Lu, Z., Afonso, C.L., Flores, E.F., Weiblen, R., Kutish, G.F., Rock, D.L. 2003. Genome of bovine herpesvirus 5. Journal of Virology. 77(19):10339-10347.

LIGATION PROPERTIES OF A NEW CLASS OF NAD-DEPENDENT DNA LIGASE FROM ENTOMOPOXVIRUS MELANOPLUS SANGUINIPES - (Peer Reviewed Journal) - (22-Mar-02)

A NOVEL SWINE VIRULENCE DETERMINANT IN THE LEFT VARIABLE REGION OF THE AFRICAN SWINE FEVER VIRUS GENOME - (Peer Reviewed Journal) - (18-Dec-01)

THE GENOME OF CULEX NIGRIPALPUS BACULOVIRUS - (Peer Reviewed Journal) - (09-Aug-01)

THE GENOME OF CULEX NIGRIPALPUS BACULOVIRUS - (Abstract Only) - (11-Mar-01)