Projects

ENGINEERING ENZYMATIC REDIRECTION OF NATURAL CROP OIL PRODUCTION TO INDUSTRIAL OIL PRODUCTION Appropriated (D)   Accession Number: 418098

TUNG SEED OIL BODY PROTEOMICS Nonfunded Cooperative Agreement (N)   Accession Number: 420768

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Expression and purification of diacylglycerol acyltransferases - (Abstract Only) - (20-Dec-12)

Molecular properties of the class III subfamily of acyl-coenyzme A binding proteins from tung tree (Vernicia fordii) - (Peer Reviewed Journal)

Pastor, S., Sethumadhavan, K., Ullah, A.H.J., Gidda, S., Cao, H., Mason, C., Chapital, D., Scheffler, B., Mullen, R., Dyer, J., Shockey, J. 2013. Molecular properties of the class III subfamily of acyl-coenyzme A binding proteins from tung tree (Vernicia fordii). Plant Science. 203-204:79-88.

Comparison of TaqMan and SYBR Green qPCR methods for quantitative gene expression in tung tree tissues - (Peer Reviewed Journal)

Cao, H., Shockey, J.M. 2012. Comparison of TaqMan and SYBR Green qPCR methods for quantitative gene expression in tung tree tissues. Journal of Agricultural and Food Chemistry. 60:12296-12303.

Glyceollins, soy isoflavone phytoalexins, improve oral glucose disposal by stimulating glucose uptake - (Peer Reviewed Journal)

Boue, S.M., Isakova, I.A., Burow, M., Cao, H., Bhatnagar, D., Sarver, J., Shinde, K.V., Erhardt, P., Heiman, M. 2012. Glyceollins, soy isoflavone phytoalexins, improve oral glucose disposal by stimulating glucose uptake. Journal of Agricultural and Food Chemistry. 60(25):6376-6382.

Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2 - (Peer Reviewed Journal)

Cao, H., Chapital, D.C., Howard Jr, O.D., Deterding, L.J., Mason, C.B., Shockey, J.M., Klasson, K.T. 2012. Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2. Applied Microbiology and Biotechnology. 96(3):711-727.

Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2 - (Peer Reviewed Journal) - (23-Dec-11)

Structure-function analysis of diacylglycerol acyltransferase sequences from 70 organisms - (Peer Reviewed Journal)

Cao, Heping. 2011. Structure-function analysis of diacylglycerol acyltransferase sequences from 70 organisms. BMC Research Notes. 4:249 (24 pages).

Expression of a lipid-inducible, self-regulating form of Yarrowia lipolytica lipase LIP2 in Saccharomyces cerevisiae - (Peer Reviewed Journal)

Shockey, J., Chapital, D., Gidda, S., Mason, C., Davis, G., Klasson, K., Cao, H., Mullen, R., Dyer, J. 2011. Expression of a lipid-inducible, self-regulating form of Yarrowia lipolytica lipase LIP2 in Saccharomyces cerevisiae. Applied Microbiology and Biotechnology. 92(6):1207-1217.

Expression of tung tree diacylglycerol acyltransferase 1 in E. coli - (Peer Reviewed Journal)

Cao, H., Chapital, D.C., Shockey, J.M., Klasson, K.T. 2011. Expression of tung tree diacylglycerol acyltransferase 1 in E. coli. BMC Biotechnology. 11:73 (13 pages).

Bioengineering recombinant diacylglycerol acyltransferases - (Book / Chapter)

Cao, H. 2011. Bioengineering recombinant diacylglycerol acyltransferases. In: Carpi, A., editor. Progress in molecular and environmental bioengineering-from analysis and modeling to technology applications. Rijeka, Croatia: InTech Open Access Publisher. p. 467-482.

Bioengineering recombinant tung tree diacylglycerol acyltransferases - (Abstract Only)

Cao, Heping, Chapital, Dorselyn C., Howard, Jr., O.D., Shockey, Jay M., Klasson, K. Thomas. 2011. Bioengineering recombinant tung tree diacylglycerol acyltransferases (abstract). Presented at the Society for Industrial Microbiology and Biotechnology Annual Meeting and Exhibition in New Orleans, Louisiana, July 24-28. http://sim.confex.com/sim/2011/webprogram/Paper19213.html.

Structure-function analysis of diacylglycerol acyltransferase sequences from tung tree and 82 other Organisms - (Abstract Only)

Cao, Heping. 2011. Structure-function analysis of diacylglycerol acyltransferase sequences from tung tree and 82 other organisms (abstract). Presented at the 2011 American Society of Plant Biologists Annual Meeting in Minneapolis, Minnesota, August 6-10. http://abstracts.aspb.org/pb2011/public/P02/P02042.html

Structure-function analysis of diacylglycerol acyltransferase sequences for metabolic engineering and drug discovery - (Abstract Only)

Cao, Heping. 2011. Structure-function analysis of diacylglycerol acyltransferase sequences for metabolic engineering and drug discovery (abstract). Presented at the Society for Industrial Microbiology and Biotechnology Annual Meeting and Exhibition in New Orleans, Louisiana, July 24-28. http://sim.confex.com/sim/2011/webprogram/Paper19779.html.

Expression and purification of membrane protein diacylglycerol acyltransferase - (Abstract Only)

Cao, H. 2011. Expression and purification of membrane protein diacylglycerol acyltransferase (abstract). Cambridge Healthtech Institute's Protein Engineering Summit 2011. p. 8.

Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2 - (Abstract Only)

Howard, O., Chapital, D.C., Shockey, J.M., Cao, H. 2011. Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2 (abstract). Southern Section of the American Society of Plant Biologists. p. 5.

Purification of recombinant tung tree diacylglycerol acyltransferases from E. coli - (Abstract Only)

Cao, H., Chapital, D.C., Howard Jr, O.D., Jiang, X., Shockey, J.M., Klasson, K.T. 2011. Purification of recombinant tung tree diacylglycerol acyltransferases from E. coli (abstract). Journal of the Federation of American Societies for Experimental Biology. 25:765.8.

Cinnamon polyphenol extract regulates tristetraprolin and related gene expression in mouse adipocytes - (Peer Reviewed Journal)

Cao, H., Anderson, R.A. 2011. Cinnamon polyphenol extract regulates tristetraprolin and related gene expression in mouse adipocytes. Journal of Agricultural Food & Chemistry. 59(6):2739-2744.

Recombinant protein production technology - (Review Article)

Cao, H. 2010. Recombinant protein production technology. Acta Agriculturae Universitatis Jiangxiensis (Natural Sciences Edition). 32(5):1018-1031.

Expression of tung seed diacylglycerol acyltransferases (DGAT) in E. coli and yeast - (Abstract Only)

Cao, H., Chapital, D.C., Mason, C.B., Shockey, J.M., Klasson, K.T. 2010. Expression of tung seed diacylglycerol acyltransferases (DGAT) in E. coli and yeast (abstract). Plant Biology 2010, July 31-August 4, Montreal, Canada.

Improving recombinant protein purification yield - (Abstract Only)

Cao, H. 2010. Improving recombinant protein purification yield (abstract). BIT Life Sciences' PepCon-2010 Conference, March 21-23, Beijing, China. p. 207.

Cinnamon and immune actions: potential role in Tristetraprolin-Mediated inflamatory diseases - (Book / Chapter)

Cao, H. 2010. Cinnamon and immune actions: Potential role in tristetraprolinmediated inflammatory diseases. In: Watson, R.R., Zibadi, S., Preedy, V.R., editors. Dietary Components and Immune Function. New York, NY: Humana Press. p. 553-565.

Potential Targets for Seed Improvement through Bioengineering Starch Synthesis Pathways - (Abstract Only)

Cao, F., Cao, H. 2009. Potential targets for seed improvement through bioengineering starch synthesis pathways (abstract). Plant Biology 2009, July 18-22, Honolulu, Hawaii.

Cinnamon extract regulates glucose transporter and insulin-signaling gene expression in mouse adipocytes - (Peer Reviewed Journal)

Cao, H., Graves, C.J., Anderson, R.A. 2010. Cinnamon extract regulates glucose transporter and insulin-signaling gene expression in mouse adipocytes. Phytomedicine. 17(3):1027-1032.

Chromium regulation of multiple gene expression in rats with high-fructose diet-induced metabolic syndrome - (Abstract Only)

Cao, H., Hininger-Favier, I., Benaraba, R., Roussel, A., Anderson, R.A. 2009. Chromium regulation of multiple gene expression in rats with high-fructose diet-induced metabolic syndrome. Meeting Abstract.

Cinnamon extract exhibits insulin-like and independent effects on gene expression in adipocytes - (Abstract Only)

Cao, H., Anderson, R.A. 2009. Cinnamon extract exhibits insulin-like and independent effects on gene expression in adipocytes. Meeting Abstract.

Tea and cinnamon polyphenols improve the metabolic syndrome - (Review Article)

Cao, H., Qin, B., Panickar, K.S., Anderson, R.A. 2008. Tea and cinnamon polyphenols improve the metabolic syndrome. Agro-Food Industry Hi-Tech. 19(6):14-17.

Green tea: molecular targets in glucose uptake, inflammation, and insulin signaling pathways - (Book / Chapter)

Cao, H., Roussel, A., Anderson, R.A. 2010. Green tea: molecular targets in glucose uptake, inflammation, and insulin signaling pathways. Comprehensive Bioactive Natural Products. Vol. 2-Efficiacy, Safety & Clinical Evaluation I, Stadium Press LLC, P. 429-448.

Quantitative evaluation of his-tag purification and immunoprecipitation of tristetraprolin and its mutant proteins from transfected human cells - (Peer Reviewed Journal)

Cao, H., Lin, R. 2009. Quantitative evaluation of his-tag purification and immunoprecipitation of tristetraprolin and its mutant proteins from transfected human cells. Biotechnology Progress. 25:461-467.

Cinnamon: Molecular evidence for the health benefits through its insulin-like and anti-inflammatory effects - (Book / Chapter)

Cao, H., Urban Jr, J.F., Anderson, R.A. 2010. Cinnamon: Molecular evidence for the health benefits through its insulin-like and anti-inflammatory effects. In: Gupta V.K., Taneja, S.C., Gupta, B.D., editors. Comprehensive Bioactive Natural Products, Vol.6 - Extraction, Isolation & Characterization. Studium Press LLC, USA. p203-227.

Cinnamon polyphenol extract affects immune responses by regulating anti- and proinflammatory and glucose transporter gene expression in mouse macrophages - (Peer Reviewed Journal)

Cao, H., Urban Jr, J.F., Anderson, R.A. 2008. Cinnamon polyphenol extract affects immune responses by regulating anti- and proinflammatory and glucose transporter gene expression in mouse macrophages. Journal of Nutrition. 138(5):833-840.

Production and characterization of ZFP36L1 antiserum against recombinant protein from Escherichia coli - (Peer Reviewed Journal)

Cao, H., Lin, R., Ghosh, S., Anderson, R.A., Urban Jr, J.F. 2008. Production and characterization of ZFP36L1 antiserum against recombinant protein from Escherichia coli. Biotechnology Progress. 24(2)326-333.

Production and Characterization of ZFP36L1 Antiserum Against Recombinant Protein From Escherichia coli - (Abstract Only)

Cao, H., Lin, R., Ghosh, S., Anderson, R.A., Urban Jr, J.F. 2008. Production and Characterization of ZFP36L1 Antiserum Against Recombinant Protein From Escherichia coli. [abstract]. Journal of Federation of American Societies for Experimental Biology. 22:1003.7.

Phosphorylation of recombinant tristetraprolin in vitro - (Peer Reviewed Journal)

Cao, H., Rui, L. 2008. Phosphorylation of recombinant tristetraprolin in vitro. Protein Journal. 27(3):163-169.

Green Tea Increases Insulin Sensitivity and Decreases Brain Oxidative Stress in Fructose Fed Rats - (Abstract Only)

Roussel, A., Hininger, I., Coves, S., Cao, H., Anderson, R.A. 2008. Green Tea Increases Insulin Sensitivity and Decreases Brain Oxidative Stress in Fructose Fed Rats. Journal of Federation of American Societies for Experimental Biology. 22:445.4.

Tristetraprolin family proteins may prevent and treat cardiovascular diseases - (Book / Chapter)

Cao, H. 2008. Tristetraprolin family proteins may prevent and treat cardiovascular diseases. In: Takeyama T., editor. Messenger RNA Research Perspectives. New York: Nova Science Publishers, Inc. pp 9-16.

Phosphorylation site analysis of the anti-inflammatory and mRNA-destabilizing protein tristetraprolin - (Review Article)

Cao, H., Deterding, L., Blackshear, P. 2007. Phosphorylation site analysis of the anti-inflammatory and mRNA-destabilizing protein tristetraprolin. Expert Review of Proteomics. 4(6):711-726.

Post-translational modifications of the mRNA destabilizing protein tristetraprolin - (Abstract Only)

Cao, H., Anderson, R.A., Blackshear, P.J. 2007. Post-translational modifications of the mRNA destabilizing protein tristetraprolin. [CD-ROM].Version ---. . Cambridge Health Tech Institute Conference. [CD-ROM].

The anit-inflammatory protein tristetraprolin is phosphorylated at multiple sites by multiple protein kinases - (Abstract Only)

Cao, H., Anderson, R.A., Blackshear, P.J. Protein Kinase Targets[CD-ROM]. Version ?, where? Cambridge Healthtech Institute.

Prevention of obesity by plant polyphenols via tristetraprolin-mediated cytokine mrna instability - (Abstract Only)

Cao, H., Urban Jr, J.F., Anderson, R.A. 2007. Prevention of obesity by plant polyphenols via tristetraprolin-mediated cytokine mrna instability. BARC Poster Day.

Insulin increases tristetraprolin and decreases VEGF gene expression in mouse 3T3-L1 adipocytes - (Peer Reviewed Journal)

Cao, H., Urban Jr, J.F., Anderson, R.A. 2008. Insulin increases tristetraprolin and decreases VEGF gene expression in mouse 3T3-L1 adipocytes. Obesity. 16:1208-1218.

Green tea polyphenol extract regulates the expression of genes involved in glucose uptake and insulin signaling in rats fed a high fructose diet - (Peer Reviewed Journal)

Cao, H., Hininger-Favier, I., Kelly, M.A., Benaraba, R., Dawson, H.D., Coves, S., Roussel, A.M., Anderson, R.A. 2007. Green tea polyphenol extract regulates the expression of genes involved in glucose uptake and insulin signaling in rats fed a high fructose diet. Journal of Agricultural and Food Chemistry. 55(15):6372-6378.

Green tea increases the antiinflammatory tristetraprolin and decreases the proinflammatory tumor necrosis factor mRNA levels in rats - (Abstract Only)

Cao, H., Kelly, M.A., Kari, F., Dawson, H.D., Coves, S., Roussel, A.M., Anderson, R.A. 2007. Green tea increases the antiinflammatory tristetraprolin and decreases the proinflammatory tumor necrosis factor mRNA levels in rats. [abstract]. Journal of Federation of American Societies for Experimental Biology. 21:358.4.

Green tea increases the anti-inflammatory tristetraprolin and decreases the pro-inflammatory tumor necrosis factor mRNA levels in rats - (Peer Reviewed Journal)

Cao, H., Kelly, M.A., Kari, F., Dawson, H.D., Urban Jr, J.F., Coves, S., Roussel, A.M., Anderson, R.A. 2007. Green tea increases the anti-inflammatory tristetraprolin and decreases the pro-inflammatory tumor necrosis factor mRNA levels in rats. Journal of Inflammation. 4(1):1-12.

Cinnamon extract and polyphenols affect the expression of tristetraprolin, insulin recepter, and glucose transporter 4 in mouse 3T3-L1 adipocytes - (Peer Reviewed Journal)

Cao, H., Polansky, M.M., Anderson, R.A. 2007. Cinnamon extract and polyphenols affect the expression of tristetraprolin, insulin recepter, and glucose transporter 4 in mouse 3T3-L1 adipocytes. Archives Of Biochemistry and Biophysics. 459:214-222.

MECHANISMS FOR INCREASED GLUCOSE TRANSPORT IN 3T3-L1 ADIPOCYTES TREATED WITH AN AQUEOUS CINNAMON EXTRACT - (Abstract Only)

Kelly, M.A., Cao, H., Schoene, N.W., Anderson, R.A. 2006. Mechanisms for increased glucose transport in 3t3-l1 adipocytes treated with an aqueous cinnamon extract. (abstract) BARC Poster Day.

PRODUCTION OF RECOMBINANT ANTIBODIES FOR LOW-ABUNDANCE PROTEINS - (Abstract Only)

Cao, H., Anderson, R.A., Blackshear, P.J. 2006. Current issues in Protein Engineering. [CD-ROM] In: Production of Recombinant Antibodies For Low-abundance proteins. Protein Engineering.

INSULIN AND CINNAMON POLYPHENOLS INCREASE THE AMOUNT OF INSULIN RECEPTOR B, GLUCOSE TRANSPORTER 4, AND ANTI-INFLAMMATORY PROTEIN TRISTETRAPROLIN IN MOUSE 3T3-L1 ADIPOCYTES - (Abstract Only)

Cao, H., Polansky, M.M., Blackshear, P.J., Anderson, R.A. 2006. Insulin and cinnamon polyphenols increase the amount of insulin receptor b, glucose transporter 4, and anti-inflammatory protein tristetraprolin in mouse 3t3-l1 adipocytes. Federation of American Societies for Experimental Biology ConferenceJournal. 20:A939, #612.3.